Table 4. Effect of fractions from D. bulbifera bulbils on HDF migration.
Sample | Dose (μg/ml) | Length between the scratches (μm) | Rate of cell migration (%) | |||||
---|---|---|---|---|---|---|---|---|
Day 0 | Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | ||
Control | - | 1428.42 ± 9.67 | 810.57 ± 7.31 | 423.25 ± 17.24 | 195.10 ± 8.65 | 43.25 ± 0.51a | 70.37 ± 1.21 | 86.34 ± 0.61a |
CHCl3 fraction | 1 | 1317.69 ± 10.97 | 690.45 ± 6.85 | 278.29 ± 5.46 | 0.00 ± 0.00 | 47.60 ± 0.52*,a | 78.88 ± 0.41*,a | 100.00 ± 0.00*,a |
3 | 1276.44 ± 2.09 | 618.37 ± 6.35 | 345.58 ± 4.58 | 0.00 ± 0.00 | 51.55 ± 0.50*,a | 72.93 ± 0.36a | 100.00 ± 0.00*,a | |
EtOAc fraction | 1 | 1307.25 ± 1.38 | 670.08 ± 10.01 | 315.79 ± 5.06 | 0.00 ± 0.00 | 49.96 ± 0.75*,a | 76.42 ± 0.38*,a | 100.00 ± 0.00*,a |
3 | 1339.11 ± 6.36 | 835.64 ± 16.41 | 400.41 ± 10.73 | 224.08 ± 9.76 | 37.60 ± 1.23* | 70.10 ± 0.80 | 83.27 ± 0.73a | |
Water fraction | 3 | 1358.95 ± 6.85 | 332.69 ± 28.03 | 0.00 ± 0.00 | 0.00 ± 0.00 | 75.52 ± 2.06*,a | 100.00 ± 0.00*,a | 100.00 ± 0.00*,a |
10 | 1311.43 ± 13.33 | 675.30 ± 21.08 | 307.59 ± 8.03 | 0.00 ± 0.00 | 48.51 ± 1.61*,a | 76.55 ± 0.61*,a | 100.00 ± 0.00*,a | |
A. vera gel | 1 | 1278.53 ± 8.29 | 872.72 ± 0.91 | 447.09 ± 10.02 | 82.38 ± 41.23 | 35.73 ± 0.07 | 67.08 ± 0.74 | 93.93 ± 3.04 |
3 | 1357.91 ± 24.70 | 825.19 ± 21.12 | 256.31 ± 0.65 | 136.23 ± 10.80 | 39.23 ± 1.56 | 81.12 ± 0.05 | 89.97 ± 0.80 |
*Statistically significant difference between the control and sample, p < 0.05 (mean ± S.E.M. of three determinations).
aStatistically significant difference between A. vera gel and the sample, p < 0.05 (mean ± S.E.M. of three determinations).