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. 2020 Dec 11;6(50):eaaz7420. doi: 10.1126/sciadv.aaz7420

Fig. 4. Artificial tethering of Mael induces repression of Brm-dependent genes.

Fig. 4

(A) Upper: Schematic representation of the λN-box B–mediated tethering system. Lower: Procedure for plasmid transfection and luciferase assays. (B) Bar graph showing relative luciferase activities of Brm KD OSC lysates and EGFP KD OSC lysates. The CG14072-luc and the Hsp70-luc reporters were used. n = 4. **P < 0.01. (C) Bar graph showing relative luciferase activities of OSC lysates upon transfection of each tethering construct. The CG14072-luc reporter was used. n = 3. **P < 0.01. (D) Bar graph showing relative luciferase activities of OSC lysates upon transfection of each tethering construct. The CG14072-luc reporter was used. n = 3. **P < 0.01. (E) Bar graph showing relative luciferase activities of OSC lysates, 48 hours after transfection of each tethering construct. The CG14072-luc and the Hsp70-luc reporters were used. n = 4. **P < 0.01. (F) Bar graph showing relative luciferase activities of OSC lysates upon KD of each factor and transfection of each tethering construct. The CG14072-luc reporter was used. **P < 0.01. (G) Bar graph showing relative luciferase activities of OSC lysates upon transfection of each construct. The CG14072-luc and the Hsp70-luc reporters were used. n = 3. **P < 0.01. (H) Bar graph showing relative luciferase activities of OSC lysates upon KD of each factor and transfection of each construct. The CG14072-luc reporter was used. n = 3. **P < 0.01. (I) Western blotting showing the levels of Panx, Mael, and Gtsf1 in the Piwi complex before and after Panx or Mael depletion. (J) Western blotting showing the levels of Panx, Mael, and Gtsf1 in the Piwi complex before and after Gtsf1 depletion. (K) Western blotting showing that Mael-Brm association was weakened by loss of Panx.