Figure 3.

TBI caused tau hyperphosphorylation and cytoplasmic retention of SET accompanied by AEP activation. (A–C) Hippocampal tissues were homogenized, and tau protein levels of pS199, pS202/pT205 (AT8), pT231 were detected by immunoblotting (A, B). Total tau level was measured (C) with actin as loading control. (D) aAEP (molecular weight at 36KD) was measured by immunoblotting. (E, F) We separate cytosolic and nuclear proteins: SET in cytoplasm (E) and in nuclei (F) were measured. (G) The interaction of AEP and SET was evaluated by Co-IP. (H) Hippocampal PP2A level and activity were tested. n=3. p value significance is calculated from a one-way ANOVA, data are represented as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control group.