Table 1. Antibodies employed in this study.
Antibodies | Specific | Type | Dilution | Sources |
PSD95 | Total PSD95 | pAb | 1/1,000 | Cell Signaling |
AEP | Legumain/Asparaginyl Endopeptidase | pAb | 1/1000 | R&D |
SET | I2PP2A | pAb | 1:1000 for WB 1:50 for IP | Santa Cruze |
PP2Ac | PP2AC | mAb | 1/1000 | Millipore |
Tau-5 | Total-tau | mAb | 1:1000 for WB | Lab Vision |
pS199 | Phosphorylated Tau at Ser199 | pAb | 1:1000 for WB | Invitrogen |
pT231 | Phosphorylated Tau at Thr 231 | pAb | 1:1000 for WB | SAB |
AT8 | Phosphorylated Tau at Ser202/Thr205 | mAb | 1:1000 for WB | Thermo |
NR1 | C-terminus (amino acids 909-938) | mAb | 1/500 | Millipore |
NR2B | C-terminus (amino acids 984-1104) | pAb | 1/500 | Millipore |
NR2A | C terminal last 200 amino acids | pAb | 1/1,000 | Abcam |
GluA1 | GluA1 protein | pAb | 1/500 | Millipore |
GluA2 | GluA2 (amino acids 175–430) | pAb | 1/500 | Millipore |
Synapsin1 | Synapsin 1 | pAb | 1/1,000 | Millipore |
Synaptophysin | Synaptophysin | mAb | 1/1,000 | Sigma |
β-actin | β-actin | mAb | 1/1,000 | Abcam |
LaminB1 | LaminB1 | pAb | 1/1,000 | Abcam |
I2PP2A, inhibitor 2 of PP2A; mAb, monoclonal antibody; pAb, polyclonal antibody; WB, Western blot; IP, immunoprecipitation.