Target gene |
Upstream sequence (5’-3’) |
Downstream sequence (5’-3’) |
IL-6 |
5’- TTGGGAAGGTTACATCAGATC-3’; |
5’- GGGTTGGTCCATGTCAATTT -3’; |
IL-8 |
5’-ATGACTTCCAAGCTGGCCGTGGCT-3’; |
5’-TCTCAGCCCTCTTCAAAAACTTCTC -3’; |
TNF-α |
5’-CCAGACCCTCACACTCAGATC-3’; |
5’- CACTTGGTGGTTTGCTACGAC -3’; |
MCP-1 |
5’- ATGCAATCAATGCCCCAGTC-3’; |
5’- TGCAGATTCTTGGGTTGTGG-3’; |
VCAM-1 |
TCTC 5’- CTTAAAATGCCTGGGAAGATGGT-3’; |
5’ - GTCAATGAGACGGAGTCACCAAT-3’; |
KLF6 |
5’-GTGACAAGGGTAATGGCGAC-3’; |
5’-ATGAGCATCTGTAAGGCTTTTCT-3’; |
LOX-1 |
5’-TTACTCTCCATGGTGGTGGTGCC-3’; |
5’-AGCTTCTTCTGCTTGTTGCC-3’; |
GAPDH |
5’- GGAGCGAGATCCCTCCAAAAT-3’; |
5’- GGCTGTTGTCATACTTCTCATGG-3’. |