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. 2020 Oct 14;12(19):19012–19021. doi: 10.18632/aging.103665

Table 1. Primer sequences.

Target gene Upstream sequence (5’-3’) Downstream sequence (5’-3’)
IL-6 5’- TTGGGAAGGTTACATCAGATC-3’; 5’- GGGTTGGTCCATGTCAATTT -3’;
IL-8 5’-ATGACTTCCAAGCTGGCCGTGGCT-3’; 5’-TCTCAGCCCTCTTCAAAAACTTCTC -3’;
TNF-α 5’-CCAGACCCTCACACTCAGATC-3’; 5’- CACTTGGTGGTTTGCTACGAC -3’;
MCP-1 5’- ATGCAATCAATGCCCCAGTC-3’; 5’- TGCAGATTCTTGGGTTGTGG-3’;
VCAM-1 TCTC 5’- CTTAAAATGCCTGGGAAGATGGT-3’; 5’ - GTCAATGAGACGGAGTCACCAAT-3’;
KLF6 5’-GTGACAAGGGTAATGGCGAC-3’; 5’-ATGAGCATCTGTAAGGCTTTTCT-3’;
LOX-1 5’-TTACTCTCCATGGTGGTGGTGCC-3’; 5’-AGCTTCTTCTGCTTGTTGCC-3’;
GAPDH 5’- GGAGCGAGATCCCTCCAAAAT-3’; 5’- GGCTGTTGTCATACTTCTCATGG-3’.