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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Biochemistry. 2020 Jun 12;59(25):2371–2385. doi: 10.1021/acs.biochem.0c00293

Figure 2:

Figure 2:

Fluorescence spectra of ANS upon binding to γS-crystallins. In each case, 50 μM of soluble protein was incubated with 750 μM of ANS in order to probe changes in hydrophobic surface exposure. UVB irradiation produces considerable hydrophobic exposure. The soluble γS-WT remaining after CuCl2 addition exhibits minimal changes in its ANS fluorescence spectrum relative to that of γS-WT. Point-variant ANS fluorescence differences are consistent with previous reports.