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. 2020 Dec 2;2020:3183519. doi: 10.1155/2020/3183519

Figure 1.

Figure 1

Tt-ME prevents LPS-induced migration abilities in RAW264.7 macrophages. (a) RAW264.7 macrophages were pretreated with Tt-ME (200 μg/ml) for 30 minutes and then treated with LPS (1 μg/ml) for the indicated period. Western blot analysis was performed to confirm MMP-2 levels. (b) RAW264.7 cells were scratched and then treated with Tt-ME for 30 minutes and then treated with LPS (1 μg/ml) for 24 h. Representative images are shown at indicated time points. (c) Results were quantified using ImageJ software, and error bars represent SD of the means of three independent experiments performed in triplicates. Data are shown as the mean ± SD of three independent experiments. ∗P < 0.01; ∗∗P < 0.05.