FIGURE 1.

Multiple alignment and phylogenetic analysis of the Vasa between Sebastes schlegelii and other species. (A) Multiple alignment was carried out using the AlignX program. The eight conserved motifs are shown in block, including I domain (AQTGSGKT), IA domain (PTREL), IB domain (GG), IC domain (TPGRL), II domain (DEAD), III domain (SAT), IV domain (ARGLD), and V domain (GRTGR). The DEAD-box sequence was indicated in bright red, while Helicase C was in rose red. (B) The phylogenetic tree of Vasa was established by MEGA4 with neighbor-joining method. The numbers adjacent to nodes indicated bootstrap percentage value for 1000 replicates.