FIGURE 7.

The ability of Oryzias melastigma and Pagrus major vasa 3′UTRs in labeling zebrafish PGCs. The EGFP-Pmvasa 3′UTR and mCherry-Omvasa 3′UTR mRNAs were co-microinjection into zebrafish embryos. Fluorescence appeared at the early gastrula stage (A1–A3) and highly expressed in the embryonic body at the early somite stage (B1–B3); PGCs did not differentiate from somatic cells at the heart beating stage (C1–C3) and 1 day after hatching (D1–D3). View of the embryos of the heart beating stage in a wide range (C2′,C3′). Scale bars, 100 μm.