TABLE 1.

Primers used in the study.

Primer	Sequence	Purpose
BrvasF	CTGATTTCATTGCCACTT	ISH probe for detecting Ssvas mRNA
BrvasR	ATTGGTGACCTTTATGGA
BrutrF	AGGAAATATTAGAGCACA	Obtaining black rockfish and zebrafish vasa 3′UTRs by RT-PCR
BrutrR	GCACAAATACTTATTTAT
ZbutrF	CTGGCCTCACACCTGTTA
ZbutrR	CACCAGTATCCGTCTTTATT
VeBrF	GAGCTGTACAAGTAAAGGAA ATATTAGAGCACA	Obtaining vasa 3′UTR fragments for Gibson assembly
VeBrR	GAATTCACTAGTGATGCACAA ATACTTATTTAT
VeZbF	GAGCTGTACAAGTAACTGGC CTCACACCTGTTA
VeZbR	ATTTCTGCCTATGACCACTA GCTTAAGGGCGCC
BrvecF	ATCACTAGTGAATTCGCG	Obtaining vector fragments for Gibson assembly
BrvecR	TTACTTGTACAGCTCGTC
ZbvecF	ATCGAATTCCCGCGGCCG
ZbvecR	CTGCTCGACATGTTCATT
T7	TAATACGACTCACTATAGGG	Preparation synthesis templates for chimeric mRNAs
BrutrR	GCACAAATACTTATTTAT
SP6	ATTTAGGTGACACTATAG
ZbutrR	CACCAGTATCCGTCTTTATT