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Single-cell RNA-sequencing reveals a cytotoxic gene expression signature in the clonally expanded, mutation harboring lymphocyte clone. (A) tSNE visualization of merged CD8+ cells with flow cytometry enriched clonally expanded CD8+ lymphocytes using Seurat-package. (B) Original identity of the cells. (C) Cell lineages using the naïve cells as the starting cluster were identified using the Slingshot tool. (D) Expression levels of selected genes over the clusters in tSNE visualization.
