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. 2020 Nov 24;11:583594. doi: 10.3389/fmicb.2020.583594

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Copyright © 2020 Mollah, Roy, Choi, Hasan, Al Baki, Yeom and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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An entomopathogenic nematode (EPN) isolate multiplied in S. exigua host. (A) Symptom of a dead insect larva infected (+) with the nematode isolate compared to an uninfected (−) healthy larva. (B) Release of infective juveniles (IJs) from the dead larva. (C) Morphological characters of IJs, including total body length from a whole view, lateral view showing excretory pore (‘Ep’), and terminal view showing tail. IJs were topically applied onto L5 larvae of S. exigua. IJs were fixed in triethanolamine formalin for observation under a phase contrast microscope.