Table 4.
Analysis of reference gene expression variability in the subcutaneous inguinal fat during the development of obesity.
| NormFinder | GeNorm | BestKeeper | Delta-Ct | Consensus | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Genes | Stability value | Genes | Stability value | Genes | SD | Genes | SD | Genes | Geometric mean of ranking values | |
| 4 w NFD/HFD (n = 8/8) |
TBP | 0.066 | PPIA | 0.175 | 18S | 0.413 | YWHAZ | 0.389 | PPIA | 2.55 |
| PPIA | 0.123 | YWHAZ | 0.175 | UBC | 0.659 | HPRT | 0.396 | TBP | 2.78 | |
| RPLP0 | 0.130 | RPLP0 | 0.185 | TBP | 0.724 | PPIA | 0.399 | YWHAZ | 3.08 | |
| HPRT | 0.167 | TBP | 0.204 | HPRT | 0.737 | RPLP0 | 0.401 | HPRT | 3.56 | |
| YWHAZ | 0.206 | HPRT | 0.237 | B2M | 0.786 | TBP | 0.404 | RPLP0 | 4.12 | |
| ACTB | 0.260 | B2M | 0.260 | ACTB | 0.797 | B2M | 0.501 | 18S | 5.62 | |
| B2M | 0.292 | ACTB | 0.295 | PPIA | 0.822 | ACTB | 0.519 | B2M | 5.96 | |
| UBC | 0.496 | GAPDH | 0.368 | RPLP0 | 0.853 | GAPDH | 0.680 | UBC | 6.00 | |
| GAPDH | 0.615 | UBC | 0.462 | YWHAZ | 0.857 | UBC | 0.692 | ACTB | 6.48 | |
| 18S | 0.777 | 18S | 0.558 | GAPDH | 1.086 | 18S | 0.941 | GAPDH | 8.71 | |
| 8 w NFD/HFD (n = 8/8) |
PPIA | 0.107 | RPLP0 | 0.159 | 18S | 0.212 | PPIA | 0.322 | PPIA | 1.68 |
| TBP | 0.123 | YWHAZ | 0.159 | PPIA | 0.363 | YWHAZ | 0.409 | RPLP0 | 3.31 | |
| HPRT | 0.198 | TBP | 0.249 | B2M | 0.385 | HPRT | 0.424 | TBP | 3.31 | |
| YWHAZ | 0.214 | PPIA | 0.303 | TBP | 0.388 | RPLP0 | 0.426 | YWHAZ | 3.46 | |
| RPLP0 | 0.221 | HPRT | 0.332 | HPRT | 0.456 | TBP | 0.433 | HPRT | 3.87 | |
| UBC | 0.264 | B2M | 0.352 | RPLP0 | 0.514 | B2M | 0.477 | 18S | 4.60 | |
| B2M | 0.271 | 18S | 0.384 | GAPDH | 0.523 | UBC | 0.517 | B2M | 5.24 | |
| 18S | 0.306 | UBC | 0.415 | UBC | 0.523 | 18S | 0.538 | UBC | 7.20 | |
| ACTB | 0.428 | ACTB | 0.456 | YWHAZ | 0.547 | ACTB | 0.557 | GAPDH | 9.15 | |
| GAPDH | 0.467 | GAPDH | 0.510 | ACTB | 0.735 | GAPDH | 0.608 | ACTB | 9.24 | |
| 12 w NFD/HFD (n = 8/8) |
HRRT | 0.120 | RPLP0 | 0.433 | GAPDH | 0.772 | HRRT | 0.629 | HRRT | 1.86 |
| PPIA | 0.239 | HPRT | 0.433 | B2M | 0.776 | PPIA | 0.645 | PPIA | 2.45 | |
| B2M | 0.289 | PPIA | 0.492 | PPIA | 0.805 | RPLP0 | 0.666 | RPLP0 | 3.03 | |
| RPLP0 | 0.331 | B2M | 0.516 | YWHAZ | 0.857 | ACTB | 0.719 | B2M | 3.46 | |
| YWHAZ | 0.354 | ACTB | 0.558 | TBP | 0.866 | YWHAZ | 0.726 | YWHAZ | 5.14 | |
| 18S | 0.386 | 18S | 0.584 | HRRT | 0.944 | B2M | 0.743 | GAPDH | 5.20 | |
| ACTB | 0.448 | YWHAZ | 0.603 | RPLP0 | 0.976 | TBP | 0.805 | ACTB | 5.79 | |
| TBP | 0.505 | TBP | 0.629 | ACTB | 1.053 | 18S | 0.807 | TBP | 6.88 | |
| GAPDH | 0.754 | GAPDH | 0.670 | 18S | 1.054 | GAPDH | 0.879 | 18S | 7.14 | |
| UBC | 1.015 | UBC | 0.869 | UBC | 2.011 | UBC | 1.478 | UBC | 10.00 | |
| 16 w NFD/HFD (n = 8/8) |
PPIA | 0.099 | B2M | 0.174 | 18S | 0.326 | PPIA | 0.403 | PPIA | 2.11 |
| RPLP0 | 0.195 | HPRT | 0.174 | GAPDH | 0.502 | YWHAZ | 0.467 | RPLP0 | 3.08 | |
| YWHAZ | 0.205 | YWHAZ | 0.255 | RPLP0 | 0.631 | RPLP0 | 0.470 | YWHAZ | 3.22 | |
| TBP | 0.228 | PPIA | 0.296 | TBP | 0.720 | HPRT | 0.472 | HPRT | 4.23 | |
| HPRT | 0.283 | RPLP0 | 0.331 | PPIA | 0.759 | TBP | 0.488 | B2M | 4.24 | |
| B2M | 0.363 | TBP | 0.344 | YWHAZ | 0.829 | B2M | 0.538 | TBP | 4.68 | |
| UBC | 0.412 | ACTB | 0.401 | UBC | 0.963 | ACTB | 0.598 | 18S | 5.33 | |
| ACTB | 0.541 | GAPDH | 0.475 | HPRT | 0.967 | GAPDH | 0.621 | GAPDH | 5.83 | |
| GAPDH | 0.552 | 18S | 0.533 | B2M | 1.008 | 18S | 0.768 | ACTB | 7.91 | |
| 18S | 0.572 | UBC | 0.588 | ACTB | 1.180 | UBC | 0.776 | UBC | 8.37 | |
| All NFD/HFD (n = 32/32) |
PPIA | 0.085 | PPIA | 0.358 | TBP | 0.710 | PPIA | 0.563 | PPIA | 1.19 |
| B2M | 0.112 | TBP | 0.358 | PPIA | 0.730 | RPLP0 | 0.651 | TBP | 2.45 | |
| RPLP0 | 0.135 | RPLP0 | 0.408 | 18S | 0.730 | TBP | 0.681 | RPLP0 | 2.91 | |
| HPRT | 0.144 | HPRT | 0.485 | RPLP0 | 0.798 | HPRT | 0.682 | B2M | 4.16 | |
| YWHAZ | 0.176 | B2M | 0.530 | B2M | 0.801 | ACTB | 0.708 | HPRT | 4.60 | |
| TBP | 0.193 | ACTB | 0.567 | GAPDH | 0.833 | B2M | 0.739 | 18S | 6.59 | |
| 18S | 0.263 | YWHAZ | 0.619 | HPRT | 0.860 | YWHAZ | 0.802 | YWHAZ | 6.65 | |
| ACTB | 0.368 | GAPDH | 0.680 | YWHAZ | 0.901 | GAPDH | 0.843 | ACTB | 6.82 | |
| UBC | 0.471 | 18S | 0.762 | ACTB | 0.980 | UBC | 1.015 | GAPDH | 7.87 | |
| GAPDH | 0.575 | UBC | 0.832 | UBC | 1.068 | 18S | 1.072 | UBC | 9.49 | |
NFD, normal-fat diet; HFD, high-fat diet; All, all four points (4 w, 8 w, 12 w, 16 w); SD, standard deviation. Gene names indicated in bold are the most appropriate selection of 4 reference genes for all comparisons.