Table 2.
B. infantis extracellular metabolite production during growth on 2′fucosyllactose is similar to that both in co-fermentation of fucose and lactose and on fucose and lactose as sole carbohydrates.
| Carbohydrate | Lactate (mM) | Acetate (mM) | Formate (mM) | 1,2-PD (mM) | Δ[Fucose] (mM) | Ace:Lac | Form:Ace | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Raw | Norm | Raw | Norm | Raw | Norm | Raw | Norm | ||||
| 0.8% 2′FL | 16 ± 1 | 16 ± 1 | 40.0 ± 0.8 | 40.0 ± 0.8 | 11.9 ± 0.5 | 11.9 ± 0.5 | 7.6 ± 0.1 | 7.6 ± 0.1 | −7.5 ± 0.4 | 2.5 | 0.3 |
| 0.8% fucose | 3.14 ± 0.08 | 18.97 ± 0.08 | 3.5 ± 0.2 | 21.3 ± 0.2 | 2.6 ± 0.5 | 15.6 ± 0.5 | 5.30 ± 0.08 | 21.01 ± 0.08 | −5.3 ± 0.9 | 1.1 | 0.7 |
| 0.8% lactose | 30.2 ± 0.5 | 22.9 ± 0.5 | 56 ± 1 | 42 ± 1 | 3.6 ± 0.2 | 2.7 ± 0.2 | ND | ND | ND | 1.8 | 0.06 |
| 0.8% fucose/0.8% lactose | 28.1 ± 0.3 | 22.4 ± 0.3 | 45.5 ± 0.7 | 36.3 ± 0.7 | 3.9 ± 0.3 | 3.1 ± 0.3 | 4.07 ± 0.05 | 3.25 ± 0.05 | −4.8 ± 0.4 | 1.6 | 0.09 |
Change in concentration is calculated using extracellular concentrations prior to growth and following stationary phase. ND, not detected.