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. 2020 Nov 24;8:581972. doi: 10.3389/fcell.2020.581972

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Copyright © 2020 Hanai, Jacob, Nakagawa, Tuan, Nakamura and Shimomura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total protein and human growth factor analysis of soluble decellularized extracellular matrix (ECM) preparations. (A) SDS-PAGE analysis of each urea-extracted tissue. (B) Growth factor concentrations (pg/ml) in 500 μg/ml soluble ECM preparations. n = 3 per condition. *p < 0.05, **p < 0.01, significant difference from each preparation. ^§§ p < 0.01, significant difference from any other preparations. bFGF, basic fibroblast growth factor; IGF-1, insulin-like growth factor-1; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1.