Figure 2.

Intensity of fluorescent stains from 48 h biofilm on cover glasses of P. aeruginosa alone, Candida alone, and P. aeruginosa + C. albicans (P. aeruginosa + C. albicans) for ECM by AF647 (red color fluorescence), bacterial nucleic acid by SYTO9 (green color fluorescence), and fungal cell wall by calcofluor white (CW2MR; blue color fluorescence) (A) with the representative fluorescent images (B) are demonstrated. There was different depth on biofilms in images of the P. aeruginosa + C. albicans group because of the prominent biofilm thickness. Mean ± SE was used for data presentation, and the differences between groups were examined by one-way ANOVA followed by Tukey’s analysis for comparisons of multiple groups or 2 groups (A). A p-value of < 0.05 was considered statistically significant. (Independent triplicate experiments were performed.) (*p<0.05; ^# p<0.05).