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. 2020 Nov 24;10:594336. doi: 10.3389/fcimb.2020.594336

Figure 6.

Figure 6

Diagram of the experimental design demonstrated lower bacteria in the biofilm preparation of simultaneous incubation of P. aeruginosa and Candida (P. aeruginosa + C. albicans) or the Candida addition in 24 h biofilm-formed bacteria (P. aeruginosa > C. albicans) in comparison with P. aeruginosa biofilms (A), crystal violet–stained biofilm in 96-well plates with representative pictures (B), fluorescent intensity of ECM by AF647 (red), bacterial nucleic acid by SYTO9 (green), and fungal cell wall by calcofluor white (CW2MR; blue) induced on cover glass biofilms and catheters (in incubator) (C, D) and organism burdens from biofilms using TSA for bacteria (E) and SDA for fungi (F) are demonstrated. Mean ± SE was used for data presentation, and the differences between groups were examined by one-way ANOVA followed by Tukey’s analysis for comparisons of multiple groups or 2 groups, respectively (B–F). A p-value of < 0.05 was considered statistically significant. (Independent triplicate experiments were performed.) (*p<0.05; # p<0.05).