Figure 4.

Trypomastigote inactivation by 405 nm blue-light in plasma and platelets. Samples were exposed to 15 mW/cm² for 2 h (108 J/cm²), 4 h (216 J/cm²), and 5 h (270 J/cm²) (A) Log reduction values of T. cruzi trypomastigotes exposed in PC (Mean ± SD: 2 h exposure = 2.9 ± 0.064, 4 h exposure = 8.4 ± 0.35, 5 h exposure = 8.5 ± 0.12). (B) Log reduction values of T. cruzi trypomastigotes exposed in plasma (2 h exposure = 0.50, 4 h exposure = 10.60, 5 h exposure = 11.10). Each point represents the data from platelets obtained from at least two independent experiments. For the parasites exposed to 405 nm light in plasma, the mean and SD values for log reduction could not be calculated because at the highest dilution (10⁻¹²) of the unexposed parasites tested in culture, >50% of the wells showed T. cruzi trypomastigote and were scored as positive wells. The titer was estimated to be ≥11 Log₁₀TCID₅₀.