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. 2020 Nov 24;10:592757. doi: 10.3389/fonc.2020.592757

Figure 5.

Figure 5

SNHG7 modulated chemoresistance and cancer cell stemness partially via miR-34a. (A) The expression of miR-34a in MCF-7/ADR cells was detected in MCF-7/ADR cells transfected with sh-NC, sh-SNHG7, sh-SNHG7+miR-34a inhibitor and sh-SNHG7+ inhibitor NC by qRT-PCR. (B–D) The IC50 of adriamycin in MCF-7/ADR cells transfected with sh-NC, sh-SNHG7, sh-SNHG7+miR-34a inhibitor and sh-SNHG7+ inhibitor NC was determined by MTT assay. (E) The percentages of CD44+/CD24- MCF-7/ADR cells transfected with sh-NC, sh-SNHG7, sh-SNHG7+miR-34a inhibitor and sh-SNHG7+ inhibitor NC was determined by flow cytometry analysis. (F) The diameters of sphere-forming cells in the sh-NC, sh-SNHG7, sh-SNHG7+miR-34a inhibitor and sh-SNHG7+ inhibitor NC groups were determined by sphere formation assay. (G) The protein expression levels of Nanog, SOX2, and OCT4 in MCF-7/ADR cells transfected with sh-NC, sh-SNHG7, sh-SNHG7+miR-34a inhibitor and sh-SNHG7+ inhibitor NC were measured by western blot. *P < 0.05 represent a statistically significant difference.