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. 2020 Nov 24;11:597433. doi: 10.3389/fimmu.2020.597433

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Copyright © 2020 Elahi, Vega-López, Herman-Miguel, Ramírez-Estudillo, Mancilla-Ramírez, Motyka, West and Oyegbami

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differential expression of ROS by CD45+ and CD45− CECs in mice. (A) Representative plots showing percentages of CD45+ subpopulation among mice splenic CECs at day-3 and day-25. (B) Cumulative data showing percentages of CD45+ CECs among splenic mice CECs from day 3 to day 25. (C) Representative plot of CD45 expressing CECs in splenic CECs of a 15-day-old mice. (D) Representative histogram of ROS expression in CD45+ versus CD45⁻CECs from the spleen of a 15-day-old mouse. (E) Cumulative data showing mean fluorescence intensity (MFI) of ROS in CD45⁺/CD45⁻CECs of neonatal mice (day 15). (F) Representative plots of CD45 expression among splenic CECs versus their counterparts in the bone marrow (BM) of 15-day-old mice. (G) Cumulative data showing percentages of CD45+ CECs in the spleen versus bone marrow on neonatal mice. (H) Representative histogram of ROS expression in total CECs in the bone marrow compared to the spleen of a day-15 mouse. (I) Cumulative data showing mean fluorescence intensity (MFI) of ROS in the bone marrow versus spleen of neonatal mice.