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. 2020 Nov 25;11:577971. doi: 10.3389/fneur.2020.577971

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Copyright © 2020 Achmus, Ruhnau, Grothe, von Sarnowski, Bröker, Dressel, Schulze and Vogelgesang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STAT3p and Arg1 were determined in myeloid-derived suppressor cell (MDSC) subpopulations of stroke patients in comparison to healthy controls. Monocytic MDSCs (Mo-MDSCs; CD11b⁺/CD14⁺/HLA-DR^dim/−) (A,D); granulocytic or polymorphonuclear MDSCs (PMN-MDSCs; CD11b⁺/CD15⁺/CD14⁻) (B,E); and early MDSCs (e-MDSC; Lin⁻/HLA-DR⁻/CD33⁺/CD11b⁺) (C,F) were measured in 10 stroke patients (black dots) in comparison to 10 healthy age-matched controls (white dots) on days 1, 3, and 5 after stroke. Arg1 (A–C) and STAT3p (D–F) were stained intracellularly and analyzed by flow cytometry (LSR II, BD). Data are provided as geometric mean fluorescence intensity (MFI). Median and interquartile range are provided.