Figure 1. FAM134B SNP association with low GlcCer plasma level (A) and skeletal muscle myosin’s effect on blood loss during tail bleeding in hemophilia A mouse models (B).
(A) The distribution of plasma GlcCer levels in VTE patients and controls with/without FAM134B rs78314670 mutation were shown. The plasma levels of GlcCer are shown as μg/mL. Solid thick lines indicate median values and the dotted line indicate the value for the 10th percentile of the control values. The difference of median values between VTE patients and controls was calculated by Mann-Whitney test and p-values were shown.
(B) Wild type C57BL/6J mice were injected with 0.25 mg/kg of anti-FVIII antibody (acquired hemophilia A model) (N=32) or vehicle (saline) (N=24) at 2 hours prior to tail cut. Then, mice were injected with vehicle (saline) or 5.4 mg/kg of rabbit skeletal muscle myosin at 15 min prior to tail cut. The distal portion of the tail was surgically removed via a scalpel blade at 1.5-mm tail diameter to induce a moderate bleeding effect. Tails were immersed in 50 mL of saline at 37°C and total blood loss per mouse weight (μL/g) was measured as the blood volume collected during 10 min following tail cut. Bars indicated median with interquartile range shown.