Figure 6.

Aspirin suppresses the BEFV-activated Src/JNK/AP-1 pathway at the early stage and reverses the BEFV-inactivated PI3K/Akt/mTOR pathway at late stage. MDBK cells were pretreated with or without aspirin (5 mM), respectively for 30 min, followed by infection with BEFV at an MOI of 1 for 2 hpi (A) and 18 hpi (B, C). The cell lysates or cytosolic and nuclear fraction were collected at the indicated time points and subjected to immunoblotting with the respective antibodies. The levels of indicated proteins in the mock control group were considered onefold. The activation and inactivation folds indicated below each lane were normalized against values for the mock control group. Protein levels were normalized to those for β‐actin. Signals in all Western blots were quantified with ImageJ software. All experiments were conducted in three independent experiments. The predicted size of each protein was labeled at the right-hand side in kDa.