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. 2020 Nov 24;11:587556. doi: 10.3389/fmicb.2020.587556

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Copyright © 2020 Ruiz-Lopez, Foster, Boothman, Cole, Morris and Lloyd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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QPCR results show the average number of copies per mL per sampling site and time (FT: feeding tank, MP: main ponds and SP: subponds). Copy numbers are expressed on log scale and error bars represent standard error. Missing bars represent lack of samples for certain sampling dates (FT and SP). A standard curve for QPCR reaction was calculated at concentration ranging from 7.53 × 10⁻⁴ to 7.530 × 10³ ng mL⁻¹ (2.8 × 10³−2.8×10¹² DNA copies) to estimate the concentration of DNA in the samples.