TABLE 1.

Distribution of samples taken for a period of 30 months from different areas within the SNF pond, and analyzed using high-throughput (Illumina) DNA microbial profiling.

Main sampling areas	Feeding tank (FT)		Main ponds (MP)				Subponds (SP)
Sampling points	Feeding tank (FT01)	Number of sequences (after QC)	Main pond 2 (MP2)	Number of sequences (after QC)	Main pond 3 (MP3)	Number of sequences (after QC)	Subpond 1 (SP1)	Number of sequences (after QC)	Subpond 2 (SP2)	Number of sequences (after QC)
Date \
October 2016	FT01 FT02	183,179 153,474	MP2_01	171,186	MP3_01	147,661	–		–	–
January 2017	–	–	–	–	–	–	SP1_01*	NA	SP2_01*	NA
June 2017	–	–	MP2_02	180,440	MP3_02	170,531	–	–	–	–
October 2017	–	–	MP2_03	110,308	MP3_03	151,014	–	–	–	–
January 2018	–	–	MP2_04	209,292	MP3_04	174,953	SP1_02	178,489	SP2_02	155,145
June 2018	–	–	MP2_05	173,003	MP3_05	163,231	SP1_03	139,082	SP2_03	175,436
November 2018	–	–	MP2_06	138,631	MP3_06	146,546	SP1_04	175,585	SP2_04	133,749
February 2019	–	–	MP2_07	156,500	MP3_07	124,047	SP1_05	150,385	SP2_05	109,585
April 2019	–	–	MP2_08	134,663	MP3_08	148,415	SP1_06	93,102	SP2_06	133,452

Samples SP1_01 and SP2_01 (*) were not sequenced using the Illumina platform but instead were analyzed using culturing techniques (with Sanger sequencing of isolated pure cultures).