Figure 1.

1,25(OH)2D3 differentially modulates integrin surface expression on activated T cells depending on their activation pathway. (A) Human CD4+ T cells were left unstimulated (US) or were activated with anti-CD3 and anti-CD28 or anti-CD46 as indicated, in the presence or absence of 1,25(OH)2D3 (VitD). After 4 days, expression of CD46, CD69, CD49d, CD29, and CD11a was assessed by flow cytometry (mean ± SEM, n= 24, one way ANOVA with Holm-Sidak’s multiple comparisons post hoc test). (B) The change in expression of CD49d, CD29, and CD11a upon activation compared to expression in unstimulated T cells was calculated (mean ± SEM, n= 22, Friedman test with Dunn’s multiple comparisons test). *: p<0.05 to 0.01, **: 0.001 to 0.01, ***: 0.0001 to 0.001, and ****: < 0.0001.