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. 2020 Nov 24;11:598727. doi: 10.3389/fimmu.2020.598727

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Copyright © 2020 Killick, Hay, Morandi, Vermeren, Kari, Angles, Williams, Damoiseaux and Astier

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Crosstalk between the CD46 and vitamin D pathways. Human CD4+ T cells were activated as indicated and expression of VDR, miR-9, CYP27B1, and CYP24A1 was quantified by qPCR after 4 days. Relative quantification was calculated according to the expression of the house keeping gene (ΔCt) and the fold change compared to unstimulated samples (2^-ΔΔCt) was calculated. Analysis was performed using one way Anova with Sidak’s multiple comparisons test. *: p<0.05 to 0.01, **: 0.001 to 0.01, ***: 0.0001 to 0.001, and ****: < 0.0001.