FIGURE 4.

(A) Influence of different framework origins on the relative interface and elbow angle distributions. The chimeric c836 antibody (PDB: 3L7E) is illustrated in blue, while the optimized humanization variant m1295 (PDB: 4PS4) is depicted in orange. The calculations of the FFT of the respective distributions are displayed, showing the angle variations occurring faster than 1 ns, between 0.1 and 10 ns, and slower than 10 ns. (B) Histograms of the respective interface and elbow angle distributions, including the respective X-ray structures of the chimeric and the humanized Fabs, which were used as starting structures for molecular dynamics simulations.