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The effect of temperature on the solubility limits of different SMs in the Ld phase. The solubility of 16:0-SM (A) and 24:0-SM (B) in the Ld phase was measured in POPC bilayers containing 0 or 20 mol % cholesterol. Above these concentrations, the SMs formed ordered domains. The values are averages of ≥3 experiments ± SD.
