Figure 2.

Overview of the strains used and resulting predominant post-translational modifications observed in tubulin purified from each strain. (A–C) The samples were primarily polyglycylated (wild-type), polyglutamylated (TTLL3(A-F)-KO), or polyglycylated only on the β-tubulin CTT (ATU1-6D). (D–F) Time-lapse images showing the trajectory of typical swimming T. thermophila cells are shown. The two mutant strains have ciliary defects and abnormal swimming patterns. To see this figure in color, go online.