Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 11;11(12):1049. doi: 10.1038/s41419-020-03244-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A The expression of E2F1 in normal cervix (NC) (n = 10), HSIL (n = 7) and squamous cell carcinoma (SCC) (n = 21) based on the data from GEO database (GSE7803). B GSEA analysis based on GSE7803 revealed the enrichment of E2F1 with high NCAPH expression. C The luciferase assay showed that the luciferase activity increased dramatically when E2F1 overexpression plasmid (pCMV-E2F1) was co-transfected with 3’-UTR regions of NCAPH gene (pGL3-NCAPH) into 293T cells. E2F1, pCMV-E2F1; NCAPH, pGL3-NCAPH; pGL3-Basic, mock vector; pCMV, mock vector. D, E, F Real-time PCR and Western Blot analysis showed that mRNA and protein levels of NCAPH decreased with the knock-down of E2F1 in HeLa and SiHa cells. NC, cells treated with negative control siRNA; siE2F1, cells treated with siRNA targeting E2F1 gene. G Overexpression of E2F1 significantly increased the protein level of NCAPH in HeLa and SiHa cells. pCMV, cells transfected with pCMV mock vector; OE-E2F1, cells transfected with pCMV-E2F1 plasmid. H, I, J Real-time PCR and Western Blot analysis showed that E2F1 and NCAPH expression decreased with the knock-down of E7 in HeLa and SiHa cells. NC, cells treated with negative control siRNA; siE7, cells treated with siRNA targeting E7 gene. K Western Blot analysis showed that the over-expression of E7 in RPE1-16E7 cells increased the expression of E2F1 and NCAPH significantly. With the interference of E2F1 expression, NCAPH level in RPE1-16E7 cells decreased dramatically. Data are presented as mean ± SD (n = 3). * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001, **** represents p < 0.0001.