Fig. 5. The activity levels of uncharacterized iModulons agree with their putative functions.

Bars and lines indicate means, black dots indicate individual samples, and error bars indicate one standard deviation. The “Other” category includes all conditions except the ones in the plot, with the number of samples included in parentheses. Vertical gray lines separate different experiments in the data set. a The activity levels of the Ybc iModulon indicate that it may be a response to heat shock or germination. The Belitsky minimal media (BMM) control occurs at 37 °C. b The activity levels of the Yrk iModulon (putative sulfur carriers) suggest that it is a response to diamide. The three conditions on the right were taken from LB cultures 10 min after exposure to the labeled stressor. c The activity levels of the WapA iModulon indicate activation by nutrient limitation (glucose exhaustion and the three growth phases of M9 media) and suppression by osmotic stress, both in the short (light blue time course) and long term (bars). “Exp”, “Tran”, and “Stat” refer to exponential, transition, and stationary phase, respectively.