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. 2020 Dec 11;10:21811. doi: 10.1038/s41598-020-76255-3

Table 4.

Formulation and proximate compositions of the experimental diets (% of dry matter).

Ingredients Diets
C0 C8 C12 C16 C20 C24
Fish meal 57 57 57 57 57 57
Wheat gluten 13 13 13 13 13 13
Alpha-starch 0 5 5 5 5 5
Corn starch 0 1.7 5.7 9.7 13.7 17.7
Soybean lecithin 1 1 1 1 1 1
Fish oil 3.5 3.5 3.5 3.5 3.5 3.5
Choline chloride 0.4 0.4 0.4 0.4 0.4 0.4
Ethoxyquin 0.05 0.05 0.05 0.05 0.05 0.05
Mold inhibitora 0.1 0.1 0.1 0.1 0.1 0.1
Monocalcium phosphate 0.5 0.5 0.5 0.5 0.5 0.5
Vitamin premixb 0.6 0.6 0.6 0.6 0.6 0.6
Minerals premixc 0.5 0.5 0.5 0.5 0.5 0.5
Y2O3 0.1 0.1 0.1 0.1 0.1 0.1
Bentonite 6.7 0 0 0 0 0
Carboxymethyl cellulose 16.55 16.55 12.55 8.55 4.55 0.55
Total 100 100 100 100 100 100
Proximate analysis (% dry matter)
Dry matter (% diet) 95.65 96.80 96.61 97.23 97.08 96.96
Crude protein 49.96 49.59 49.49 49.60 49.99 50.44
Crude lipid 9.59 9.73 9.65 9.98 9.75 9.96
Carbohydrated 0.93 7.01 11.06 15.60 19.96 23.18
Ash 17.73 12.46 11.72 11.73 11.93 12.13

aMold inhibitor: 50% calcium propionic acid and 50% fumaric acid.

bVitamin premix (g kg−1 of diet): microcrystalline cellulose, 9.884 × 10–2; VA, 1.92 × 10–4; VB1, 1.5 × 10–4; VB2, 2.7 × 10–4; VB6, 1.2 × 10–4; VB12, 6 × 10–5; VD, 2.1 × 10–4; VE, 1.44 × 10–3; VK, 6 × 10–5; calcium pantothenate, 3.6 × 10–4; nicotinic acid, 1.2 × 10–3; folic acid, 1.2 × 10–4; biotin, 3.6 × 10–4; inositol, 4.8 × 10–3; VC phosphate, 1.2 × 10–2.

cMineral premix (g kg−1 of diet): MgSO4·7H2O, 6 × 10–3; CuSO4·5H2O, 5 × 10–5; FeSO4·H2O, 4 × 10–4; ZnSO4·H2O, 2.5 × 10–4; MnSO4·H2O, 2.25 × 10–4; CoCl2·6H2O (1%), 2.5 × 10–4; Na2SeO3 (1%), 1 × 10–4; calcium iodate, 3 × 10–4; zeolite powder, 4.243 × 10–2.

dDetermined by the 3,5-dinitro salicylic acid method120.