Table 5.
List of PCR primer pairs used for the real-time PCR analysis.
| Genes | Forward (5′–3′) | Reverse (5′–3′) | Accession no. |
|---|---|---|---|
| myf5 | GCAACGCCATCCACTACATCG | TGCATTCAACTGGTGCCACACT | DQ872515 |
| myod | GCAACGCCATCAGCTACATCG | CGTTTGGAGTCTGGGAGAAATAAG | DQ184914 |
| myog | GTCTGGGGGTGTTGGAGTTGG | GACGCCTCTTCTCCCTCATCG | EF144128 |
| mrf4 | AGAGCAGCGGGGAGGAACAC | GACCTTGCAGGCCCACATGA | MK453386 |
| mstn | TTTGAGGACTTTGGCTGGGACT | GCGACATCTTGGTGGGGGTA | DQ412048 |
| igf-I | CTGTGCACCTGCCAAGACTA | CTTTGTGCCCTGCGGTACTA | MK453382 |
| igf-II | ATCAAAGCACAGGAGCAGGCAATC | TGTCCGTGGCGAGCAAGACG | MK453383 |
| igf1r | GAAGGGCGTGGTCAAAGATG | AGGTCGGAGGGAGCGTAAGT | MK453384 |
| igf2r | TCCGCTGGTACACGTCCTAC | GGTGAGCCCTGATCCGATAT | MK453385 |
| murf-1 | TTGTGCCGTAGTTGTGCTAGTGAC | CATGGCGATCAAGCACGACCTC | MK292717 |
| mafbx | GCTGGGTGAAAACCGAGGAG | CTTCTTGGCAGCCATGTCGT | MK453387 |
| capn1 | CATCGTAGACGGAGCCACTC | GACCGTGAGGAACCACTCTG | MK292720 |
| capn2 | AAAGTGAACGGCTGCTACGA | TCGTAGTTCTCAGCGATGCC | MK453381 |
| ctsd | ACGTGCACAATCGGAGACTT | GATGTTGTCAAAGACCGGCG | FJ172450 |
| ctsl | CTCCTGCTGGTCCTTCAGTTCAAC | ACGATGTAGCGGAAGGCATTGTC | FJ172449 |
| opa1 | CAGTGGCCGAGAGTTTGACC | TCACCGTACTGATGACGCCT | MK757585 |
| mfn1 | CGGTATTGGCCACACCACTA | AGAGCCCTCTGTCTTGAGGT | MK757584 |
| mfn2 | TGGTGACAGGTCTTGCATCC | CAACCCACTGCCTTCCAGAT | MK757586 |
| tnf-α | GTCCTGGCGTTTTCTTGGTA | CTTGGCTCTGCTGCTGATTT | AB040448 |
| il-6 | CTCCAGTCGAATACGAGCCC | ACTCTTTCTGGTGGTGAGCG | DQ884914 |
| gysm | GAGGAGCACATAGCAGACCC | TTACACGACTCATCGACCGC | MN201568 |
| pygm | AACAATGACCGAGTGGTGGG | TTCTCAGCCAGAGTGACACG | MN201569 |
| β-actin | GGAAATCGTGCGTGACATTAAG | CCTCTGGACAACGGAACCTCT | HQ386788 |