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. 2020 Dec 11;6:40. doi: 10.1038/s41531-020-00143-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Quantitative RT-PCR analysis shows decreased Gch1 expression in human α-synuclein transgenic flies at 10 days of age when compared to control flies. Neuronal knockdown of Gch1 enhances α-synuclein induced (b) climbing and (c) locomotor activity deficits when compared to flies expressing α-synuclein alone. d Quantitative RT-PCR analysis shows the knockdown level of Gch1 in the two RNAi lines used. Dietary folate supplementation in flies partially rescues α-synuclein induced (e) climbing and (f) locomotor activity deficits when compared to flies expressing α-synuclein not receiving folate. Control genotype: nSyb-QF2, nSyb-GAL4/+. All data are represented as mean ± SEM. For more than two groups, data were analyzed using one-way ANOVA with Tukey post-hoc test for multiple groups. For two groups, data are analyzed using two-tailed student’s t test. *p < 0.05, **p < 0.01, ***p < 0.005, n = 6. Flies are 10 days old in a–c and e, f, and 1 day old in d.