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. 2020 Dec 11;10:21779. doi: 10.1038/s41598-020-78711-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Absorption spectroscopy. (A) Spectra corresponding to purified RBD produced in HEK-293T cells (black), P. pastoris clones 1 (blue) and 7 (red), and a simulated RBD spectrum (green) (27.5 μM) calculated from its composition of aromatic amino acid (15 Phe, 15 Tyr, and 2 Trp in RBD). (B) Fourth derivative spectra corresponding to the aromatic amino acids (Tyr (blue), Trp (violet), Phe (yellow)) and a simulated RBD spectrum (green). (C) Comparison between the fourth derivative spectra from RBD obtained in HEK-293T (black), P. pastoris RBD Clone 7 (red) and the simulated spectrum presented in A (green).