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. 2020 Dec 11;10:21779. doi: 10.1038/s41598-020-78711-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hydrodynamic behavior of RBD. (A) SEC-HPLC of RBD produced in P. pastoris (red), HEK-293T (black) and molecular weight markers (dashed green line). This analysis was carried out by injecting 50 μL protein aliquots (0.70 and 0.75 mg/mL for RBD produced in P. pastoris and HEK-293T, respectively) in 20 mM Tris–HCl, 100 mM NaCl, pH 7.0 buffer. The inset shows the correlation between molecular weight and elution volume obtained from the molecular weight markers: (1) gammaglobulin (158 kDa), (2) ovoalbumin (44 kDa), (3) myoglobin (17 kDa), and (4) vitamin B12 (1350 Da). (B) Deconvolution analysis of the chromatographic profile from RBD from P. pastoris. The experimental profile (red), deconvolution of the peak in two different gaussian curves (green and yellow) and the sum of the deconvoluted peaks (blue) are compared.