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. 2020 Dec 10;27:9–16. doi: 10.1016/j.jot.2020.10.011

Figure 2.

Figure 2

HGF promoted osteoclastogenesis in vitro. Cells were cultured with RANKL alone (50 ​ng/mL) or M-CSF (30 ​ng/mL) plus RANKL (50 ​ng/mL) for 3d or 5d. Representative TRAP staining images of (a) RAW264.7 ​cells, (b) BMMs with osteoclast quantitation (n ​= ​6). RANKL ​+ ​HGF: stimulated with HGF (50 ​ng/mL) for 3 ​d; Scale bars, 100 ​μm. Total mRNA was respectively isolated from (c) RAW264.7 ​cells and (d) BMMs. SU11274: stimulated with HGF (50 ​ng/mL) and SU11274 (0.5 ​μM) for 5d (n ​= ​3). Relative expression of nfatc1, acp5, and ctsk mRNA were tested. β-actin mRNA was detected as internal control (n ​= ​3). Data are expressed as means ​± ​SD; ∗p ​< ​0.05. Experiments were repeated 3 times, and similar results were obtained.