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. 2020 Dec 11;10:214. doi: 10.1186/s13568-020-01163-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Expression and identification of recombinant PCV3 capsid proteins expressed in E. coli. a The SDS-PAGE analysis of the recombined capsid protein of PCV3. M, protein marker; 1, control bacteria with pET-28a; 2, the bacteria with pET-3CAP; 3, the bacteria with pET-Δ3CAP; 4, the bacteria with pET-3CAP-O; 5, the bacteria with pET-Δ3CAP-O. b Western blots analysis of the expressed recombined proteins using the anti-His antibody. The blot corresponds to lanes 1–5 from (a). The arrows represent the location of the recombined ΔCAP (25 kDa), respectively. The expression of the recombined CAP protein did not be detected