Fig. 1.

Timeline of the behavioral tests performed and validation of mutant mice. a All cohorts of mice were acclimated to the behavior testing apparatus for 2 days, 4 h each, before starting experiments. The baseline readings for grimacing behavior, paw withdrawal in response to von Frey filaments, paw withdrawal in response to heat stimuli, paw temperature, and paw edema were recorded before injection of CFA into the left hind paw, which was designated as the ipsilateral paw. Experimental readings for the affective behaviors were recorded at 4 h, day 1, 2, 3, 5, and 7 after the injection. CFA, complete Freund’s adjuvant. b Representative agarose gel showing genotypes of WT, eIF4E^S209A−/−, and eIF4E^S209A+/− mice. c eIF4E protein is not phosphorylated in the eIF4E^S209A cohorts. d Total eIF4E protein levels in tested tissues from all cohorts show no differences across age and genotypes