Fig. 3.

Aged eIF4E^S209A demonstrate reduced spontaneous pain. Graphs depicting grimace at indicated times post CFA injection in young (a) and aged (b) mice. Data are plotted as means with standard error (young WT, n = 7; young eIF4E^S209A, n = 10; aged WT, n = 21; aged eIF4E^S209A, n = 8). For a and b, two-way repeated measures ANOVA with Sidak’s post hoc test was performed. ns, not significant, **p = 0.0066. c Effect size for all four cohorts compared with baseline. Two-way ANOVA was performed with Tukey’s post hoc. *p 0.0191, ****p < 0.0001. BL, baseline; 4 h, four hours; D1, day 1; D2, day 2; D3, day 3; D5, day 5; D7, day 7; CFA, complete Freund’s adjuvant