Fig. 4.

Aged and young eIF4E^S209A mice show faster resolution of evoked pain. Graphs depicting ipsilateral paw withdrawal in response to Von Frey filaments at indicated times post CFA injection in young (a) and aged (b) mice. Data are plotted as means with standard error (young WT, n = 7; young eIF4E^S209A, n = 10; aged WT, n = 21; aged eIF4E^S209A, n = 8). The dashed gray line represents the mean contralateral paw withdrawal for filament applied for all experimental animals. Two-way repeated measures ANOVA with Sidak’s post hoc test was performed for multiple comparisons between genotypes for young and aged animals. ns, not significant, ***p = 0.0003, ****p < 0.0001. c Effect size for all four cohorts. Two-way ANOVA was performed with Tukey’s post hoc for multiple comparisons between age and genotypes. **p 0.0046, ****p < 0.0001. Graphs depicting paw withdrawal latencies in response to heat applied at ipsilateral paw at indicated times post CFA injection in young (d) and aged (e) mice. Data plotted and analyzed as stated for parts a and b. ns, not significant, *p 0.0177 for young mice, *p 0.0153 for aged mice. The dashed gray line represents the mean contralateral paw withdrawal latency in response to heat applied for all experimental animals. f Effect size for all four cohorts compared with baseline. Data plotted and analyzed as for c. *p 0.0417 for eIF4E^S209A mice and **p 0.0376 for aged mice. BL, baseline; 4 h, four hours; D1, day 1; D2, day 2; D3, day 3; D5, day 5; D7, day 7; CFA, complete Freund’s adjuvant