Figure 3.

GCAT1 interacts with the PTBP1 and hnRNPK proteins

(A) Detection of GCAT1-binding proteins by RNA pull-down assays. Two specific bands of ~60 kDa (red box) were pulled down by GCAT1 and subsequently identified by mass spectrometry. (B) Western blot showing the specific association between GCAT1 and PTBP1 and hnRNPK in the samples obtained from the RNA pull-down. The antisense transcript of GCAT1 was used as the negative control. (C and D) Confirmation of the interaction between GCAT1 and PTBP1 and hnRNPK by RIP using the PTBP1 antibody and hnRNPK antibody in KGN cells. Results are expressed as the mean ± SD (n = 3). ∗p < 0.05 by two-tailed Student’s t test.