GCAT1 regulated GC proliferation and G1/S transition via p27
(A) The CCK8 assay showed the cell viability of KGN cells after GCAT1 silencing, CDKN1B silencing, and co-silencing. Results are expressed as the mean ± SD (n = 3). ∗∗∗p < 0.001 by two-tailed Student’s t test. (B and C) EdU staining assay showing the proliferation ability of KGN cells after GCAT1 silencing, CDKN1B silencing, and co-silencing. Results are expressed as the mean ± SD (n = 3). ∗∗∗p < 0.001 by two-tailed Student’s t test. (D) The flow cytometry cell cycle distribution of KGN cells after GCAT1 silencing, CDKN1B silencing, and co-silencing. Results are expressed as the mean ± SD (n = 3). ∗p < 0.05 by two-tailed Student’s t test.