Figure 2.

Effect of endothelial miR-150 deletion on development of PH in Sugen/hypoxia mice

(A) Effect of tamoxifen administration on miR-150 levels in lungs of wild-type (WT) (150^+/+) and miR-150iEC-HTKO mice (150^+/−). Data are expressed as fold change of normoxia control. (B–D) RVSP (B), RV/LV+S (C), and percentage (D) of muscularized vessels <50 μm in diameter/total number of vessels in the lungs of normoxia wild-type control (+ tamoxifen), normoxia miR-150^+/− control (without tamoxifen), and Sugen/hypoxia miR-150^+/− mice with and without tamoxifen, as indicated. In (A)–(D), open bars mark miR-150-deficient animals. (E) Representative images of α-SMA staining. Scale bar, 25 μm. ∗p < 0.05, ∗∗p < 0.005, ∗∗∗∗p < 0.0001, comparisons with normoxia control; ^#p < 0.05, ^####p < 0.0001, comparisons with miR-150^+/− control (by one-way ANOVA with a Tukey’s post-test). Bars are means ± SEM. n = 4–8 mice/group.