Figure 4.

Endothelium-protective effects of miR-150 and PTPMT1

(A–D) Effect of miR-150 mimic and miR-150 inhibitor on (A) miR-150 expression levels in HPAECs, (B) starvation-induced apoptosis (caspase-3/7 activity assay), (C) hypoxia-induced proliferation (EdU incorporation assay), and (D) hypoxia (24 h)-induced NF-κB activity (luciferase reporter assay). (E–H) Effect of PTPMT1 overexpression (PTPMT1) or silencing (siPTPMT1) on (E) PTPMT1 mRNA expression, (F) apoptosis, (G) proliferation, and (H) hypoxia-induced NF-κB activity in HPAECs. In (A), n = 3; in (B)–(H), n = 6. ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, comparisons with untreated transfection control; ^#p < 0.05, ^##p < 0.001, ^####p < 0.0001, comparisons with treated (starvation or hypoxia, as appropriate) transfection controls (one-way ANOVA with a Tukey’s post-test). Bars are mean fold changes of transfection control ± SEM.