Figure 7.

miR-150, PTPMT1, and cardiolipin levels and mitochondrial biogenesis in IPAH ECFCs

(A and B) miR-150 (A) and PTPMT1 (B) expression levels in ECFCs from healthy individuals and IPAH patients (fold change of control). (C–E) Cardiolipin levels (C), mitochondrial fragmentation (D), and mitochondrial content (mitochondrial coverage/cell) (E) in ECFCs treated, as indicated. (F) Representative images of mitochondrial fragmentation in healthy and IPAH ECFCs. Inset in the top right corner is an enlarged image of the boxed area. Mitochondria were immunolabeled with fluorescein isothiocyanate (FITC) (green) and F-actin with tetramethylrhodamine isothiocyanate (TRITC)-phalloidin (red). Scale bar, 10 μm. Data are expressed as means ± SEM. ∗p < 0.05, ∗∗p < 0.01, comparison with healthy control. In (A) and (B), n = 12–14; in (C)–(E), n = 4.