Table 1.
Mutations Found in Candidate Gene Identified by RNA-Seq Transcriptome Analysis
| Chr. | Gene | Annotation | Position | Variant Calling |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Parental Strain |
Resistant Mutants |
||||||||||
| WT | A1 | B1 | C1 | D1 | E1 | F1 | G1 | ||||
| V | TGGT1_285200 | CPSF3 | 2395898 | E545K (GAG to AAG) | E545K (GAG to AAG) | E545K (GAG to AAG) | |||||
| V | TGGT1_285200 | CPSF3 | 2395976 | S519C (AGC to TGC) | |||||||
| V | TGGT1_285200 | CPSF3 | 2396165 | G456S (GGC to AGC) | G456S (GGC to AGC) | ||||||
| V | TGGT1_285200 | CPSF3 | 2396549 | Y328H (TAC to CAC) | |||||||
Amino acid substitutions with the corresponding codons shown in parentheses are indicated for each mutagenized T. gondii-resistant mutant strain.