Fig. 5.

Polydatin attenuates diabetes-induced myocardial NF-κB activation. a-c Western blot analysis of I-κB-α expression and its phosphorylation in the cytosol fractions, and nuclear translocation of p65 in the nuclear fraction of the heart tissue in different groups after respective treatments. d-g Real-time PCR analysis of IL-1β, IL-6, TNF-α and VCAM-1 mRNA level in each group of rats. h-k The expression of IL-1β, IL-6, TNF-α and VCAM-1 was determined by ELISA as described in Methods. Results were expressed as means ± SEM (n = 7). *P < 0.05, **P < 0.01 versus Con; #P < 0.05, ##P < 0.01versus DM