Fig. 6.

Polydatin prevents NADPH oxidase activity in HG-induced H9c2 cells. H9c2 cells were cultured in DMEM containing 30 mmol/L glucose (HG) or 5.5 mmol/L glucose (normal glucose) and treated with polydatin (0–40 μmol/L) for 24 h. a Cell viability was measured by a Cell Counting Kit-8 assay. b Cytotoxicity was detected via lactate dehydrogenase (LDH) assay. c NADPH oxidase activity was measured by lucigenin chemiluminescence’s assay. d Intracellular superoxide anion levels were detected in H9c2 cells using fluorogenic probe DCFH-DA. e, f and g Summary of real-time PCR data showing levels of NOX2, NOX4, and NOX1 mRNA in H9c2 cells. Data were expressed as means ± SEM (n = 6–7). *P < 0.05, **P < 0.01 versus control values; #P < 0.05, ##P < 0.01versus HG